為解決越來越多的強極性藥物和小生物分子的分離難題,賽分公司開發(fā)了一系列弱酸性、中性和堿性HILIC(Hydrophilic Interaction Chromatography)固定相來分離具有強極性的堿性、中性和酸性化合物。該類固定相是以高純度具有良好機械穩(wěn)定性的硅膠為基質(zhì),采用高純度的鍵合試劑,可最大限度實現(xiàn)硅膠表面覆蓋,從而確保了固定相的穩(wěn)定性。通過運用獨有的勻漿裝填技術裝填得到的HILIC柱柱床密度均一穩(wěn)定,可保證具有最高的柱效。
賽分公司的HILIC固定相類型包括Polar-100、Polar-Diol、Polar-Silica、Polar-Pyridine和Polar-Imidazole。Polar-100和Polar-Diol是中性的極性固定相,后者的極性要高于前者。Polar-Silica是弱酸性固定相。Polar-Pyridine和Polar-Imidazole是堿性固定相,后者的堿性要強于前者。
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | |
硅膠: | 球形, 高純度(金屬雜質(zhì)<10 ppm) | ||||
---|---|---|---|---|---|
孔徑: | 120 Å | ||||
粒徑: | 1.8、2.2、3、4、5和10 µm | ||||
孔體積: | 1.0 mL/g | ||||
比表面積: | 300 m²/g | ||||
固定相結(jié)構(gòu): | 三官能團單分子層未封尾 | ||||
碳載量: | 0.0% | 16.0% | 8.8% | 8.0% | 8.6% |
鍵合密度: | 0 | 3.0 µmol/m² |
色譜柱: HILIC, 4.6 mm I.D.×150 mm, 5 µm
流動相: CH3CN: 10mM醋酸銨緩沖液=90:10 (v/v)
流速: 1.0 mL/min
檢測波長: 254nm
進樣量: 1 µL
溫度: 室溫
樣品: 1.尿嘧啶, 2.腺苷(Adenosine), 3.尿苷(Uridine), 4.胞苷(Cytidine), 5.鳥苷(Guanosine)
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | ||
---|---|---|---|---|---|---|
長度×內(nèi)徑mm x mm | 粒徑(µm) | 訂貨號 | ||||
30 × 2.1 | 3 | 130003-2103 | 131583-2103 | 133333-2103 | 134253-2103 | 135333-2103 |
50 × 2.1 | 3 | 130003-2105 | 131583-2105 | 133333-2105 | 134253-2105 | 135333-2105 |
100 × 4.6 | 3 | 130003-4610 | 131583-4610 | 133333-4610 | 134253-4610 | 135333-4610 |
150 × 4.6 | 3 | 130003-4615 | 131583-4615 | 133333-4615 | 134253-4615 | 135333-4615 |
250 × 4.6 | 3 | 130003-4625 | 131583-4625 | 133333-4625 | 134253-4625 | 135333-4625 |
30 × 2.1 | 5 | 130005-2103 | 131585-2103 | 133335-2103 | 134255-2103 | 135335-2103 |
50 × 2.1 | 5 | 130005-2105 | 131585-2105 | 133335-2105 | 134255-2105 | 135335-2105 |
100 × 4.6 | 5 | 130005-4610 | 131585-4610 | 133335-4610 | 134255-4610 | 135335-4610 |
150 × 4.6 | 5 | 130005-4615 | 131585-4615 | 133335-4615 | 134255-4615 | 135335-4615 |
250 × 4.6 | 5 | 130005-4625 | 131585-4625 | 133335-4625 | 134255-4625 | 135335-4625 |
150 × 10.0 | 5 | 130005-10015 | 131585-10015 | 133335-10015 | 134255-10015 | 135335-10015 |
250 × 10.0 | 5 | 130005-10025 | 131585-10025 | 133335-10025 | 134255-10025 | 135335-10025 |
150 × 21.2 | 5 | 130005-21215 | 131585-21215 | 133335-21215 | 134255-21215 | 135335-21215 |
250 × 21.2 | 5 | 130005-21225 | 131585-21225 | 133335-21225 | 134255-21225 | 135335-21225 |
150 × 30.0 | 5 | 130005-30015 | 131585-30015 | 133335-30015 | 134255-30015 | 135335-30015 |
250 × 30.0 | 5 | 130005-30025 | 131585-30025 | 133335-30025 | 134255-30025 | 135335-30025 |
150 × 10.0 | 10 | 130009-10015 | 131589-10015 | 133339-10015 | 134259-10015 | 135339-10015 |
250 × 10.0 | 10 | 130009-10025 | 131589-10025 | 133339-10025 | 134259-10025 | 135339-10025 |
150 × 21.2 | 10 | 130009-21215 | 131589-21215 | 133339-21215 | 134259-21215 | 135339-21215 |
250 × 21.2 | 10 | 130009-21225 | 131589-21225 | 133339-21225 | 134259-21225 | 135339-21225 |
150 × 30.0 | 10 | 130009-30005 | 131589-30015 | 133339-30015 | 134259-30015 | 135339-30015 |
250 × 30.0 | 10 | 130009-30010 | 131589-30025 | 133339-30025 | 134259-30025 | 135339-30025 |
Polar-Silica | Polar-100 | Polar-Diol | Polar-Pyridine | Polar-Imidazole | |||
---|---|---|---|---|---|---|---|
長度×內(nèi)徑mm x mm | 粒徑(µm) | 包裝 | 訂貨號 | ||||
10 × 2.0 | 3 | 1/pk | 130003-2001 | 131583-2001 | 133333-2001 | 134253-2001 | 135333-2001 |
10 × 4.0 | 3 | 1/pk | 130003-4001 | 131583-4001 | 133333-4001 | 134253-4001 | 135333-4001 |
10 × 2.0 | 5 | 1/pk | 130005-2001 | 131585-2001 | 133335-2001 | 134255-2001 | 135335-2001 |
10 × 4.0 | 5 | 1/pk | 130005-4001 | 131585-4001 | 133335-4001 | 134255-4001 | 135335-4001 |
10 × 2.0 | 3 | 2/pk | 130003-2001T | 131583-2001T | 133333-2001T | 134253-2001T | 135333-2001T |
10 × 4.0 | 3 | 2/pk | 130003-4001T | 131583-4001T | 133333-4001T | 134253-4001T | 135333-4001T |
10 × 2.0 | 5 | 2/pk | 130005-2001T | 131585-2001T | 133335-2001T | 134255-2001T | 135335-2001T |
10 × 4.0 | 5 | 2/pk | 130005-4001T | 131585-4001T | 133335-4001T | 134255-4001T | 135335-4001T |
10 × 2.0 | 3 | 5/pk | 130003-2001F | 131583-2001F | 133333-2001F | 134253-2001F | 135333-2001F |
10 × 4.0 | 3 | 5/pk | 130003-4001F | 131583-4001F | 133333-4001F | 134253-4001F | 135333-4001F |
10 × 2.0 | 5 | 5/pk | 130005-2001F | 131585-2001F | 133335-2001F | 134255-2001F | 135335-2001F |
10 × 4.0 | 5 | 5/pk | 130005-4001F | 131585-4001F | 133335-4001F | 134255-4001F | 135335-4001F |
10 × 21.2 | 5 | 1/pk | 130005-21201 | 131585-21201 | 133335-21201 | 134255-21201 | 135335-21201 |
10 × 21.2 | 10 | 1/pk | 130009-21201 | 131589-21201 | 133339-21201 | 134259-21201 | 135339-21201 |
產(chǎn)品名稱 | 包裝(規(guī)格) | 訂貨號 |
---|---|---|
分析型保護柱套 | 10mm x2.0(4.0)mm | 102000-AH |
PEEK二接 | 7cm | 102002-P355 |
扳手 | 2個 | 102000-WRENCH |
制備型保護柱套 | 10mm x21.2mm | 102000-21201 |