應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):生物化學(xué)(Biochemistry)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):
方法(Method):
Column: |
Opalshell-C18 ( 2.6 µm, 90 Å, 4.6 x 100 mm ) |
Mobile phase: | A: ACN : 0.1 M NaAc = 5 : 95 (v/v), pH=6.5; B: 80% ACN |
Flow rate: |
1.0 mL/min |
Temperature: |
36oC |
Detection: |
UV 254nm |
Injection volume: |
0.5 µL |
Sample: | Derivatization of 18 amino acids with PITC |