應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):生物化學(xué)(Biochemistry)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Proteomix
方法(Method):
Column: |
Proteomix SCX-NP5 ( 5 µm, 4.6 x 250 mm ) |
Mobile phase: | A: 16 mM MES + 10 mM MOPS + 12 mM TAPS + 10 mM CAPSO + 30 mM NaCl, pH 5.2 by NaOH; B: 10 mM MES + 12 mM MOPS + 14 mM TAPS + 16 mM CAPSO + 30 mM NaCl, pH 10.2 by NaOH |
Flow rate: |
1.0 mL/min |
Temperature: |
25℃ |
Detection: |
UV 280 nm |
Injection volume: |
5 µL |
Sample: | H/K ( 2.7 mg/mL ) |