應用編號(Application ID):
樣品分類(Sample Category):生物化學(Biochemistry)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Antibodix
方法(Method):
Column: |
Antibodix® WCX-NP5 ( 5 µm, 4.6 x 250 mm ) |
Mobile phase: |
A: 4 mM Tris, 4 mM imidazole, 4 mM piperazine( pH 8.0 ), B: A at pH 10.83 |
Flow rate: |
0.8 mL/min |
Temperature: |
25℃ |
Detection: |
UV 280 nm |
Injection volume: |
10 µL |
Sample: |
about 5 mg/mL IgG2 |