應(yīng)用編號(Application ID):
樣品分類(Sample Category):生物化學(xué)(Biochemistry)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):Amethyst
方法(Method):
Column: |
Amethyst C18-H, 5 µm, 120 Å, 4.6 x 250 mm |
|
Mobile phase: |
A: 0.05 M Sodium Acetate B: Acetonitrile : water = 50 : 50 ( v/v ) |
|
Time( min ):
|
0.01 0.5 11 18 25 30 32 55 55.01 100 | |
B%:
|
37 32 32 50 67 69 95 95 37 37 | |
Temperature: |
Room temperature |
|
Flow rate: |
0.8 mL/min |
|
Detection: |
UV 360 nm |
|
Injection Volume: |
10 µL |
|
Sample: |
DNFB derivatived amino acids(二硝基氟苯衍生化氨基酸) |
|
1. Asp(門冬氨酸) |
2. Glu(谷氨酸) 4. His(組氨酸) 6. Gly(甘氨酸) 8. Pro(脯氨酸) 10. Val(纈氨酸) 12. Ile(異亮氨酸) 14. Phe(苯丙氨酸) 16. Lys(賴氨酸) |