應(yīng)用編號(Application ID):
樣品分類(Sample Category):生物化學(xué)(Biochemistry)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Proteomix
方法(Method):
Column: |
Proteomix-SAX, 5 µm, 4.6 x 250 mm |
Mobile phase: |
A: 20 mM Tris, pH 8.0 B: A + 1 M NaCl |
Time( min ):
|
0 3 8 30 35 |
B%:
|
0 0 40 100 100 |
Flow rate: |
0.5 mL/min |
Detection: |
UV 260 nm |
Temperature: | 30 ℃ |
Injection Volume: |
10 µL |
Sample: |
Oligonucleotide primer(寡聚核苷酸引物),10 µM each in water 1. 5'-CTGGTACGCCGACCCCGTAGCACGC-3' 2. 5'-ATATCTACACGGCTACCCGTACCAATGCTGCTTCC-3' 3. 5'-ATAAGTACCCCCTCCCCTTTATTCT-3' 4. 5'-AGGAGTACCCCCTCCCCTTTATTCT-3' 5. 5'-ACGATAGCTGATAGTCCCTTACAGC-3' 6. 5'-ACGACAGCTGATAGTCCCTGACAGC-3'
|