應用編號(Application ID):PR0088
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):Sapphire
方法(Method):
Column: |
Sapphire C18, 5 µm, 100 Å, 4.6×250 mm |
Mobile phase: |
ACN : 0.5% TEA ( adjust pH to 2.5 by H3PO4) = 13 : 87 ( v/v ) |
Flow rate: |
0.8 mL/min |
Temperature: |
30 oC |
Detection: |
UV 278 nm |
Injection volume: |
5 µL |
Sample: |
0.1 mg/mL theobromine (可可堿) in 0.2% NaOH |