應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):離子交換(Ion-exchange chromatography)
產(chǎn)品系列(Product Line):Antibodix
方法(Method):
Column: |
Antibodix WCX-NP10, 10 µm, 4.6 x 250 mm |
Mobile phase: |
A: 10 mM Sodium phosphate buffer, pH 7.5 B: A + 0.1 M NaCl |
Time( min ): | 0 30 35 40 41 50 51 80 |
A%: | 90 55 35 35 0 0 90 90 |
B%: | 10 45 65 65 100 100 10 10 |
Flow rate: |
0.8 mL/min |
Temperature: |
30 oC |
Detection: |
UV 214 nm |
Injection volume: |
50 µL |
Sample: |
1.0 mg/mL antibody |