應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):GP
方法(Method):
Column: |
GP C18 ( 5 µm, 120 Å, 4.6x 250 mm ) |
Mobile phase: |
dissolve 2.0 g of tetradecylammonium bromide R and 2.0 g of tetraheptylammonium bromide R in a mixture of 440 mL of water R, 55 mL of 0.067 M phosphate buffer solution pH 7.0 R, 5.0 mL of citrate buffer solution pH 5.0 prepared by dissolving 20.17 g of citric acid R in 800 mL of water R, adjusting to pH 5.0 with strong sodium hydroxide solution R and diluting to 1000.0 mL with water R, and 500 mL of acetonitrile R |
Flow rate: |
1.5 mL/min |
Column temperature: |
25 ℃ |
Detection: |
UV 254 nm |
Injection volume: |
20 µL |