應(yīng)用編號(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):HP
方法(Method):
Column: |
HP C18 ( 5 µm, 120 Å, 4.6 x 250 mm ) |
Mobile phase: |
A: [ H2O : 3.12% NaH2PO4 : 8%TBAOH = 576 : 200 : 24, pH 5.5 by H3PO4] : ACN = 800 : 200, B: [ H2O : 3.12% NaH2PO4 : 8%TBAOH = 126 : 200 : 24, pH 5.5 by H3PO4] : ACN = 350 : 650 |
Flow rate: |
1.0 mL/min |
Column temperature: |
40 ℃ |
Detection: |
UV 220 nm |
Injection volume: |
20 µL |