應(yīng)用編號(hào)(Application ID):
樣品分類(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產(chǎn)品系列(Product Line):Bio
方法(Method):藥典
Column: |
Bio-C18 ( 5 µm,300 Å, 4.6 x 250 mm ) |
|
Mobile phase: |
A: 0.022 M Tetramethylammonium Hydroxide ( adjust pH to 2.5 by H3PO4 ): Acetontrile= 9 : 1 ( v/v ) , |
|
Time( min ):
|
0 21 | |
B%:
|
35 57 | |
Flow rate: |
1.0 mL/min |
|
Temperature: |
40 ℃ |
|
Detection: |
220 nm |
|
Injection volume: |
100 µL |
|
Sample: |
16.7 μg/mL Calcitonin (鮭降鈣素)in 0.1 M NaH2PO4 |