應(yīng)用編號(hào)(Application ID):
樣品分類(lèi)(Sample Category):醫(yī)藥(Pharmaceutical)
分離模式(Separation Mode):正相(Normal phase chromatography)
產(chǎn)品系列(Product Line):HP
方法(Method):
Column: |
HP-Silica, 3 µm, 120 Å, 4.6 x 150 mm |
Mobile phase: |
1-Chlorobutane : THF : Methanol : Acetic acid : H2O = 890 : 56 : 28 : 24 : 0.4 ( v/v ) |
Flow rate: |
1.5 mL/min |
Temperature: |
25 ℃ |
Detection: |
UV 254 nm |
Injection volume: |
5 µL |
Sample: |
2.0 mg/mL Hydrocortisone (氫化可的松) in 1-Chlorobutane : THF : Methanol : Acetic acid ( 81.5 : 10 : 8 : 0.5, v/v ) |